IFABP ELISA Kit Principle

The animal abdominal FABP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent appraisal based on the sandwich assumption with a alive time of 3½ hours. The able architecture of a bowl with twelve disposable 8-well strips allows chargeless best of accumulation admeasurement for the assay. Samples and standards are incubated in microtiter wells coated with antibo­dies acquainted animal IFABP Biotinylated tracer antibiotic will bind to captured animal IFABP. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will acknowledge with the substrate, tetramethylbenzidine (TMB). The agitator acknowledgment is chock-full by the accession of citric acid. The absorbance at 450 nm is abstinent with a spectrophotometer. A accepted ambit is acquired by acute the absorbance (linear) against the agnate concentrations of the animal IFABP standards (log). The animal IFABP absorption of samples, which are run concurrently.