Anti-NF-κBp50（p50 NF-kappa B;p50NFKB）

Specification:100ul/200ul/1ml 
Description:
The Cayman Chemical NF-kB (human p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate. NF-kB contained in a nuclear extract, binds specifically to the NF-kB response element. NF-kB (p50) is detected by addition of specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. The Cayman Chemical NF-kB (human p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).